Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking

Abstract

A new way of deblocking of synthetic hexachlorofluorescein-oligodeoxyribonucleotides (HEX-ODN) prevents the modification of the label and the accumulation of difficult to separated by-product. It was shown that this method increases the efficiency of purification HEX-ON and the sensitivity of DNA diagnostics.

Keywords

• hexachlorofluorescein lаbel
• oligodeoxyribonucleotides
• hplc
• real time pcr