dCas9 regulator to neutralize competition in CRISPRi circuits

Hsin-Ho Huang; Massimo Bellato; Yili Qian; Pablo Cárdenas; Lorenzo Pasotti; Paolo Magni; Domitilla Del Vecchio

doi:10.1038/s41467-021-21772-6

Nature Communications (Mar 2021)

dCas9 regulator to neutralize competition in CRISPRi circuits

Hsin-Ho Huang,
Massimo Bellato,
Yili Qian,
Pablo Cárdenas,
Lorenzo Pasotti,
Paolo Magni,
Domitilla Del Vecchio

Affiliations

Hsin-Ho Huang: Department of Mechanical Engineering, Massachusetts Institute of Technology
Massimo Bellato: Laboratory of Bioinformatics, Mathematical Modelling and Synthetic Biology, Department of Electrical, Computer and Biomedical Engineering, University of Pavia
Yili Qian: Department of Mechanical Engineering, Massachusetts Institute of Technology
Pablo Cárdenas: Department of Biological Engineering, Massachusetts Institute of Technology
Lorenzo Pasotti: Laboratory of Bioinformatics, Mathematical Modelling and Synthetic Biology, Department of Electrical, Computer and Biomedical Engineering, University of Pavia
Paolo Magni: Laboratory of Bioinformatics, Mathematical Modelling and Synthetic Biology, Department of Electrical, Computer and Biomedical Engineering, University of Pavia
Domitilla Del Vecchio: Department of Mechanical Engineering, Massachusetts Institute of Technology

DOI: https://doi.org/10.1038/s41467-021-21772-6
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 7

Abstract

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CRISPRi allows for the simultaneous control of many genes, however the sgRNAs compete for binding to dCas9. Here the authors design a dCas9 concentration regulator to allow independent regulation of multiple genes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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