Transcriptomic Analysis of Salt Stress Response in <i>Pleurotus ostreatus</i>

Abstract

This study was conducted to confirm the expression patterns of genes involved in stress resistance by comparing the expression patterns of genes expressed after sodium chloride (NaCl) treatment in Pleurotus ostreatus (PO) cultivation. To confirm this, as a result of checking different gene expressions for the untreated group and the NaCl 1% and 2% treated group, a total of 12,460 gene expression differences were confirmed. There were 275 and 397 genes with increased expression in the 2.0% and 1.0% NaCl treated group and 400 and 247 genes with reduced expression in the 1.0% and 2.0% NaCl treated group, respectively. Among the genes whose expression was confirmed in DEG, qRT-PCR was performed on six genes to confirm the expression pattern of the genes affecting the mycelium structure. The DEG results showed that a putative aldo-keto reductase of akor3, Alpha-1,4 glucan phosphorylase of PLEOSDRAFT_1058949, and heme-thiolate peroxidase of HTP1 were up-regulated and that glycoside hydrolase family 92 protein of PLEOSDRAFT_1063499 were down-regulated, and the qRT-PCR showed the same results. However, hydrophobin of Hydph16 and pleurotolysin B of plyB were up-regulated in the qRT-PCR results while down-regulated in the DEG results. From the above results, it is judged that NaCl ultimately inhibits growth by inhibiting the formation of the skeleton constituting the mycelium and the physiological activity within the cell.

Keywords

• <i>Pleurotus ostreatus</i>
• sodium chloride (NaCl)
• DEGs
• qRT-PCR