Staged developmental mapping and X chromosome transcriptional dynamics during mouse spermatogenesis

Abstract

The transcriptional regulation of murine spermatogenesis is not well understood. Here, the authors use single-cell and bulk RNA-Sequencing of juvenile and adult mice to characterise somatic and germ cell development, and chromatin profile the X chromosome to show that spermatid-specific genes are repressed by H3K9me3 during meiosis.