In Vivo Analgesic Potential in Swiss Albino Mice and in Vitro Thrombolytic and Membrane Stabilizing Activities of Methanolic Extract From Suaeda Maritima Whole Plant

Abstract

Objective: Cold methanolic extract of Suaeda maritima have been considered to screen their biological properties such as analgesic, membrane stabilizing and thrombolytic activity. Although some investigations have previously been reported with this plant, the whole plant was not studied yet to date. In the present study we aimed to investigate in vivo and in vitro bioactivities of Suaeda maritima.Material and Methods: The pharmacological analgesic activity was studied by acetic acid induced writhing method in vivo in a mouse model. Membrane stabilizing activity was assessed by inhibition of haemolysis at hypotonic solution induced condition in vitro, and thrombolytic activity was evaluated by employing Streptokinase as standard in vitro.Results: Extract of Suaeda maritima at a dose of 500 mg/kg body weight showed most significant inhibition of writhing at a rate of 82.25% in mice. Among all the fractions, crude methanol extract (ME) showed maximum effect with a value of 61.01% inhibition of hemolysis at 10 mg/ml dose, when standard acetyl salicylic acid (0.10 mg/mL) revealed 66.37% inhibition of hemolysis. When clots were treated with 100 μl, moderate clot lysis activity observed with each of the test sample, however highest effect was found at 10 mg/ml dose (49.13%).Conclusion: The Suaeda maritima extract showed a remarkable analgesic effect on a mouse model and membrane stabilizing and trombolytic activities in vivo models. It may introduce a new dimension in human model in analgesic, membrane stabilizing and antitrombolytic activities.

Keywords

• suaeda maritima
• writhing
• membrane stabilizing
• thrombolytic