A semi-virtual two dimensional gel electrophoresis: IF–ESI LC-MS/MS

Stanislav Naryzhny; Victor Zgoda; Artur Kopylov; Elena Petrenko; Аlexander Archakov

doi:10.1016/j.mex.2017.08.004

MethodsX (Jan 2017)

A semi-virtual two dimensional gel electrophoresis: IF–ESI LC-MS/MS

Stanislav Naryzhny,
Victor Zgoda,
Artur Kopylov,
Elena Petrenko,
Аlexander Archakov

Affiliations

Stanislav Naryzhny: Orekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Pogodinskaya 10, Moscow, 119121, Russia
Victor Zgoda: Orekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Pogodinskaya 10, Moscow, 119121, Russia
Artur Kopylov: Orekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Pogodinskaya 10, Moscow, 119121, Russia
Elena Petrenko: Orekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Pogodinskaya 10, Moscow, 119121, Russia
Аlexander Archakov: Orekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Pogodinskaya 10, Moscow, 119121, Russia

DOI: https://doi.org/10.1016/j.mex.2017.08.004
Journal volume & issue: Vol. 4, no. C
pp. 260 – 264

Abstract

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A method for increasing the productivity of ESI LC-MS/MS (electrospray ionization-liquid chromatography-tandem mass spectrometry) was proposed and applied. After IF (isoelectric focusing) of the sample using IPG (immobilized pH gradient) strip, the strip was cut to sections, and every section was treated according to trypsinolysis protocol for MS/MS analysis. The peptides produced were further analyzed by ESI LC-MS/MS. The procedure allows to: • identify many more proteins and proteoforms compared to shotgun analysis of extracts. • build a semi-virtual 2DE map of identified proteins.

A semi-virtual two dimensional gel electrophoresis: IF–ESI LC-MS/MS

Published in MethodsX

ISSN: 2215-0161 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science
Website: http://www.journals.elsevier.com/methodsx/

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