The effects of ciprofloxacin on male rabbits: Biochemical and histopathological study

Abstract

The present study was designed to investigate the possible effect of ciprofloxacin on blood serum enzymes activities (AST, ALT, and ALP), total cholesterol, total protein, albumin, and globulin as well as the histopathological changes on liver, kidney, and testes. The study conducted on twelve male rabbits divided into two equal groups. G1 was administrated distilled water orally and considered as control group. G2 was given 100 mg/kg B.W/day of ciprofloxacin for 35 days orally. Blood samples were taken from animals after 35 days of treatment for biochemical analysis to estimate; total cholesterol (TC), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphates (ALP), total protein, albumin, and globulin, then all animals were sacrificed, liver, kidney, and testes were taken and fixed in 10% formalin for histological examination. The results showed that ciprofloxacin administration significantly (p< 0.05) increased the serum total cholesterol, AST, ALT and ALP concentration. While there were significant (P<0.05) decreased the serum level of total protein and globulin, but no significant changes were observed on the serum level of albumin. While histopathologically the liver show vacuolation of hepatocytes and congestion of central vein. The kidney display vacuolation of cortical areas and dilatation of tubules. While the testes exhibit suppression of spermatogenesis in treated animals compared with the control group. These results revealed the toxic effect of ciprofloxacin on the liver, kidney, and testes of male rabbits.