Frontiers in Plant Science (Feb 2024)

Development of KASP markers assisted with soybean drought tolerance in the germination stage based on GWAS

  • Qianru Jia,
  • Miaomiao Zhou,
  • Yawen Xiong,
  • Yawen Xiong,
  • Junyan Wang,
  • Donghe Xu,
  • Hongmei Zhang,
  • Xiaoqing Liu,
  • Wei Zhang,
  • Qiong Wang,
  • Xin Sun,
  • Huatao Chen,
  • Huatao Chen

DOI
https://doi.org/10.3389/fpls.2024.1352379
Journal volume & issue
Vol. 15

Abstract

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Soybean [Glycine max(L.)Merr.] is a leading oil-bearing crop and cultivated globally over a vast scale. The agricultural landscape in China faces a formidable challenge with drought significantly impacting soybean production. In this study, we treated a natural population of 264 Chinese soybean accessions using 15% PEG-6000 and used GR, GE, GI, RGR, RGE, RGI and ASFV as evaluation index. Using the ASFV, we screened 17 strong drought-tolerant soybean germplasm in the germination stage. Leveraging 2,597,425 high-density SNP markers, we conducted Genome-Wide Association Studies (GWAS) and identified 92 SNPs and 9 candidate genes significantly associated with drought tolerance. Furthermore, we developed two KASP markers for S14_5147797 and S18_53902767, which closely linked to drought tolerance. This research not only enriches the pool of soybean germplasm resources but also establishes a robust foundation for the molecular breeding of drought tolerance soybean varieties.

Keywords