Implantation of the collagen membrane with adsorbed multipotent stem cells in experiment

Abstract

Objective. To follow-up the processes, taking place with autologous multipotent mesenchymal stem cells (ММSC) of the bone marrow origin after their implantation into the organism on surface of the collagen material in experiment. Маterials and methods. The changes in subcutaneous adipose cellular tissue in rats after implantation of the collagen membrane with passively adsorbed on the MMSC on the surface with transfected gene GFP and additionally stained cellular membranes with Vybrant® CM-Dil were studied up, using the light microscopy methods and a luminescence. Results. While application of a luminescence in a one week after implantation of the collagen material with the MMSC adsorbed in a neighborhood with the implant there were revealed typical very small vessels owing a one-layer membrane, which have fluoresced intensely while a rodamine filter was installed. On the second week on periphery of the collagen material and in neighborhood to the implant on background of a rodamine filter application only small inclusions inside macrophages were shining with a red color. Subsequently step by step the quantity of such shining objects have reduced down to solitary ones, presenting very rarely, as well as their fluorescence intensity. Conclusion. The main cause for the tissue vascularization enhancement around the implanted collagen with the MMSC adsorbed on the surface is their differentiation into cellular elements of vascular membranes. Starting from the second week all the MMSC undergo destruction and are phagocytized by macrophages, also in this term the structures from these MMSC disappear as a result of their differentiation, including the vessels.

Keywords

• collagen; multipotent mesenchymal stem cells; implantation; аngiogenesis; macrophages