Site-specific incorporation of citrulline into proteins in mammalian cells

Santanu Mondal; Shu Wang; Yunan Zheng; Sudeshna Sen; Abhishek Chatterjee; Paul R. Thompson

doi:10.1038/s41467-020-20279-w

Nature Communications (Jan 2021)

Site-specific incorporation of citrulline into proteins in mammalian cells

Santanu Mondal,
Shu Wang,
Yunan Zheng,
Sudeshna Sen,
Abhishek Chatterjee,
Paul R. Thompson

Affiliations

Santanu Mondal: Department of Biochemistry and Molecular Pharmacology, UMass Medical School
Shu Wang: Department of Chemistry, Boston College
Yunan Zheng: Department of Chemistry, Boston College
Sudeshna Sen: Department of Biochemistry and Molecular Pharmacology, UMass Medical School
Abhishek Chatterjee: Department of Chemistry, Boston College
Paul R. Thompson: Department of Biochemistry and Molecular Pharmacology, UMass Medical School

DOI: https://doi.org/10.1038/s41467-020-20279-w
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Citrullination of arginine is crucial for several physiological processes. Here the authors report the site-specific incorporation of citrulline into proteins in mammalian cells using an engineered tRNA synthetase/tRNA pair and a photocaged-citrulline.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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