Formation of 1-O-2′-hydroxyalkyl glycerophosphatides from 1,2-heptadecanediol in myelinating brain

Abstract

1,2-Heptadecanediol-2-14C was administered intracerebrally to 18-day-old rats, and its incorporation, after 8 hr, into the individual aliphatic moieties of the ethanolamine glycerophosphatides was determined.Much of the radioactivity was found in a lipid fraction identified as 1-O-2′-hydroxyheptadecyl glycerol. Evidence is presented that a major portion of the precursor was incorporated into 1-O-2′-hydroxyheptadecy1-2-acyl ethanolamine phosphatides.Some of the diol administered was degraded to palmitic acid. The palmitic acid-1-14C derived from 1,2-heptadecanediol-2-14C apparently served as precursor for stearic and oleic acids, which were found as acyl groups, and for the biosynthesis of the corresponding O-alkyl and O-alk-1-enyl glycerols.The data presented prove that biological dehydration of 1-O-2′-hydroxyalkyl glycerophosphatides to the corresponding plasmalogens does not occur in myelinating brain.

Keywords

• ethanolamine phosphatides
• alkyl acyl glycerols
• alk-1-enyl acyl glycerols
• diacyl glycerols
• intracerebral injection
• 1,2-heptadecanediol-2-14C