Improving the Precision of Base Editing by Bubble Hairpin Single Guide RNA

Zhiwei Hu; Yannan Wang; Qian Liu; Yan Qiu; Zhiyu Zhong; Kangdi Li; Wenhua Li; Zixin Deng; Yuhui Sun

doi:10.1128/mBio.00342-21

mBio (Apr 2021)

Improving the Precision of Base Editing by Bubble Hairpin Single Guide RNA

Zhiwei Hu,
Yannan Wang,
Qian Liu,
Yan Qiu,
Zhiyu Zhong,
Kangdi Li,
Wenhua Li,
Zixin Deng,
Yuhui Sun

Affiliations

Zhiwei Hu: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), School of Pharmaceutical Sciences, Wuhan University, Wuhan, People’s Republic of China
Yannan Wang: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), School of Pharmaceutical Sciences, Wuhan University, Wuhan, People’s Republic of China
Qian Liu: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), School of Pharmaceutical Sciences, Wuhan University, Wuhan, People’s Republic of China
Yan Qiu: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), School of Pharmaceutical Sciences, Wuhan University, Wuhan, People’s Republic of China
Zhiyu Zhong: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), School of Pharmaceutical Sciences, Wuhan University, Wuhan, People’s Republic of China
Kangdi Li: Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of China
Wenhua Li: Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of China
Zixin Deng: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), School of Pharmaceutical Sciences, Wuhan University, Wuhan, People’s Republic of China
Yuhui Sun: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), School of Pharmaceutical Sciences, Wuhan University, Wuhan, People’s Republic of China

DOI: https://doi.org/10.1128/mBio.00342-21
Journal volume & issue: Vol. 12, no. 2

Abstract

Read online

Base editors are double-strand-break (DSB)-free genome editing tools and have been widely used in diverse living systems. However, it is reported that these tools can cause substantial off-target editing.

Published in mBio

ISSN: 2161-2129 (Print); 2150-7511 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/mbio

About the journal