Environmental Health and Toxicology (Dec 2014)

Sub-acute toxicity study in female ICR mice following repetitive intramuscular injection of cervical cancer vaccines

  • Seol-Hee Moon,
  • Du-Yeol Kim,
  • Jung-Min Lee,
  • Hee-Won Park,
  • Hye-Yeong Lee,
  • Yong-Hoon Lee,
  • Jaesung Lee,
  • Jiwon Jung,
  • Min-ju Kim,
  • Kyoung-Baek Choi,
  • Yu-Kyoung Oh,
  • Young-Bong Kim,
  • Sujeong Kim,
  • Seung Min Oh

DOI
https://doi.org/10.5620/eht.e2014024
Journal volume & issue
Vol. 29

Abstract

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Objectives The sub-acute toxic effects following repetitive intramuscular injection of two cervical cancer vaccines newly developed against human papillomaviruse (HPV)16/58/18 and HPV16 were investigated in female ICR (CrljOri: CD1) mice, and the no-observedadverse- effect-level (NOAEL) of the cervical cancer vaccines was estimated. Methods Female ICR mice (n=15 in each group) were exposed to a 1:1 mixture of two cervical cancer vaccines by repetitive intramuscular injection (once a week, 5 times) for 5 weeks. Mortality, body weight, organ weight, hematological/biochemical parameters, and histopathological effects were examined at different concentrations (0, 1×108, 5×108, and 2.5×109 copies/animal) of the cervical cancer vaccines. Results The cervical cancer vaccines did not show toxic responses for body weight, absolute/ relative organ weight, hematological/biochemical parameters, or histopathological parameters. Conclusions Female ICR mice exposed to vaccines for cervical cancer did not show any toxic response. We suggest that a NOAEL of the vaccine following repetitive intramuscular injection for 5 weeks is >2.5×109 copies/animal.

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