Proteins sequence analysis of contagious caprine pleuropneumonia

Abstract

A total of twenty (20) contagious bovine pleuropneumonia (CCPP) proteins were retrieved from the GenBank (www.ncbi.nlm.nih.gov). The proteins sequences were used to investigate the molecular identity of various CCPP proteins. The physico-chemical properties of CCPP proteins were performed using protparam tool. Isoelectric point (pI), molecular weight (MW), extinction coefficient (EC); instability index (II), aliphatic index (AI) and grand average of hydropathicity (GRAVY) were computed. The study revealed that the pI of CCPP proteins were acidic and basic in nature. The EC and II of CCPP proteins indicate better stability which is an indication of resistant to mutation and thermally stable. The GRAVY of CCPP proteins revealed some are positive while some are negative. The positive value indicates solubility (hydrophilic) in water while negative is not soluble (hydrophobic) in water. The amino acid composition of CCPP proteins indicates that they are rich in isoleucine, leucine and lysine. The three dimensional structures (3D) of the CCPP proteins were determine using Phyre2 server. The amino acid sequences of CCPP proteins were subjected to secondary structure prediction using ExPASy's SOPMA tool. The proteins are more of alpha helix structure. The genetic information emanating from this study may bring insight into mutagenesis and pharmacogenetic.

Keywords

• protein
• caprine pleuropneumonia
• sequence