Evaluation of usefulness of single-strand conformation polymorphism method for rapid detection of rifampicin-resistant mycobacterium tuberculosis

Abstract

The aim was to evaluate the Single-Strand Conformation Polymorphism method as a potential tool for rapid detection of rifampicin-resistant strains by the use of 39 rifampicin-resistant Mycobacterium tuberculosis strains isolated in Serbia. SSCP analysis on acrylamide gel detected 56.4% of the rifampicin-resistant M. tuberculosis strains and showed the inability to detect one of the most frequent mutations, TCG®TTG mutation in codon 531 of the rpoB gene, which was shown by automated sequencing.

Keywords

• polymorphism
• single-standed conformational
• mycobacterum tuberculosis
• rifampin
• drug resistance
• microbial