Morphological assessment of pathological changes epidermis in proliferative inflammatory skin diseases

Abstract

Background. The morphometric method is a unique method that allows obtaining the most accurate quantitative characteristics of the cellular composition of the epidermis and its layers, necessary for objectification in differential diagnosis and understanding of pathomorphological changes in the skin. The morphometric method is a unique method that allows obtaining the most accurate quantitative characteristics of the cellular composition of the epidermis and its layers, necessary for objectification in differential diagnosis and understanding of pathomorphological changes in the skin. The purpose of the research was to study pathomorphological changes in the epidermal layers of the skin using morphometric research methods for nosologies with different etiopathogenesis. Materials and methods. A morphometric study of 105 skin biopsies was carried out, of which 25 biopsies were the skin of persons without a history of chronic pathology, including skin, 22 biopsies of psoriatic papules in the progressive and stationary stages, 20 biopsies of papules of the typical form of lichen ruber planus, 19 biopsies of acne papules and vasculitis. The number of rows of spiny, granular layers of the epidermis was studied by counting the nuclei strictly arranged in one line, at the smallest distance from the basal to the stratum corneum in 5 different fields of view with the calculation of the average. The number of cells per 1 area in the thorny and granular layers was determined by ocular insertion in 5 different fields of view with the calculation of the average [Avtandilov G.G., 1990]. Results. The features of pathomorphological changes of the epidermis in the area of papules in various proliferative and inflammatory skin diseases were revealed. The most marked pathomorphological changes of the epidermis were in psoriasis. In acne, the predominance of the thickness of the thorny layer was observed. The increase in the number of rows of the granular layer was the same and less significant in acne, vasculitis and lichen ruber planus. Conclusion. The use of morphometric analysis methods in dermatology makes it possible to analyze more accurately about the severity of pathomorphological changes in the epidermis and expands the scope of practical work in terms of diagnosis and prediction of the course of the disease.

Keywords

• morphometric method
• psoriasis
• lichen ruber planus
• vasculitis
• acne