HIGH-SENSITIVITY TEST SYSTEMS BASED ON CONJUGATES «POLYMER MICROSPHERES – BIOLIGANDS» FOR RAPID PROTEINOPATHY DIAGNOSTICS

Abstract

The main approaches to the creation of highly sensitive test systems based on polymeric microspheres with immobilized bioligands intended for rapid diagnostics of proteinopathy were considered. The effect of these test systems is based on the latex agglutination reaction between the antigens grafted to the surface of polymeric microspheres and antibodies contained in a biological sample. The issues related to obtaining and optimal properties of polymeric microspheres, which must contain functional groups reactive toward bioligands at their surface were analyzed. Problems associated with immobilization of bioligands on the surface of the polymeric microspheres and the possibility to improve the composition and preparation of test systems were considered. Special attention is given to obtaining test systems for the diagnosis of thyroid disease by means one of its antigens – thyroglobulin.

Keywords

• test systems, rapid diagnosis, proteinopathy, latex agglutination, functionalized polymeric microspheres, bioligand, immobilization, thyroglobulin.