Immobilization of oxidoreductases on gold and silver nanoparticles

Abstract

Nanoparticles of silver and gold were synthesized by chemical methods. Nanoparticles of silver were obtained by reducing of silvernitrate with glucose and nanoparticles of gold – by reducing of tetrachloroauric acid trihydrate with sodium citrate or boro­hydride. New methods of functionalization of nanoparticles surface were proposed. To activate nanoparticles, two approaches were applied: nanoparticles of silver and gold synthesized by citrate method were treated with 16-mercaptohexadecanoic acid, and nanoparticles of gold obtained with the help of sodium borohydride method, were functionalized with cysteamine. Bionanomaterials were obtained by immobilizing of horse radish peroxidase and glucose oxidase of Penicillium adametzii on the activated nanoparticles. Scanning electron microscopy and spectrophotometric methods proved nanosizes of resulting nanoparticles conjugated with enzymes. The study of catalytic properties of enzymes immobilized on the nanoparticles showed that nanoparticles of gold are the most effective for peroxidase, whereas the nanoparticles of silver are optimal carriers for conjugation with glucose oxidase.

Keywords

• nanoparticles of silver or gold
• peroxidase
• glucose oxidase
• immobilization on nanoparticles