Molecular detection of Mycoplasma spp. from camel’s milk

Abstract

The dromedary camels are essential because of their multiple uses for transport, wool, meat, and milk production. Camel’s milk is considered exceptional nutrition, so it uses for therapeutic and high nutrition. Mastitis may affect camel’s milk production, cause significant economic loss, and is associated with zoonotic disease. The study aimed to detect the prevalence of Mycoplasma spp. especially M. bovis in camel’s milk using the polymerase chain reaction (PCR) method as a primary molecular diagnostic technique. Fifty milk samples were collected from she camels suffering from subclinical mastitis in Iraq. The result of the current study declared that 26.66% of the camel’s milk samples were positive for Mycoplasma. In contrast, the prevalence rate of Mycoplasma (M.) bovis in all the samples was 61.53%. The study concludes that Mycoplasma and especially M. bovis are considered one of the bacteria that cause subclinical mastitis in camels, and using the PCR method is regarded as a more rapid, simple, and current for detecting the Mycoplasma bacteria.

Keywords

• mycoplasma
• m. bovis
• pcr method
• camel’s milk