Morphological control enables nanometer-scale dissection of cell-cell signaling complexes

Liam P. Dow; Guido Gaietta; Yair Kaufman; Mark F. Swift; Moara Lemos; Kerry Lane; Matthew Hopcroft; Armel Bezault; Cécile Sauvanet; Niels Volkmann; Beth L. Pruitt; Dorit Hanein

doi:10.1038/s41467-022-35409-9

Nature Communications (Dec 2022)

Morphological control enables nanometer-scale dissection of cell-cell signaling complexes

Liam P. Dow,
Guido Gaietta,
Yair Kaufman,
Mark F. Swift,
Moara Lemos,
Kerry Lane,
Matthew Hopcroft,
Armel Bezault,
Cécile Sauvanet,
Niels Volkmann,
Beth L. Pruitt,
Dorit Hanein

Affiliations

Liam P. Dow: Mechanical Engineering and Biomolecular Science and Engineering, University of California
Guido Gaietta: Scintillon Institute
Yair Kaufman: Mechanical Engineering and Biomolecular Science and Engineering, University of California
Mark F. Swift: Scintillon Institute
Moara Lemos: Institut Pasteur, CNRS UMR3528, Structural Studies of Macromolecular Machines in Cellulo Unit
Kerry Lane: Mechanical Engineering and Biomolecular Science and Engineering, University of California
Matthew Hopcroft: Mechanical Engineering and Biomolecular Science and Engineering, University of California
Armel Bezault: Institut Pasteur, CNRS UMR3528, Structural Studies of Macromolecular Machines in Cellulo Unit
Cécile Sauvanet: Institut Pasteur, CNRS UMR3528, Structural Studies of Macromolecular Machines in Cellulo Unit
Niels Volkmann: Scintillon Institute
Beth L. Pruitt: Mechanical Engineering and Biomolecular Science and Engineering, University of California
Dorit Hanein: Scintillon Institute

DOI: https://doi.org/10.1038/s41467-022-35409-9
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

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Micropatterning on electron microscopy grids enables control of cell positioning and arrangement. Here, the authors describe detailed nanoscale characterization of a micropatterning workflow for reproducible molecular characterization through cryo-ET

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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