Local application benefits of DL-methionine as wound powder: A novel approach for wound healing process in rabbits

Abstract

This study examines the clinical and histopathological aspects of using Dl-Methionine to enhance wound healing. The purpose of wound management is enhancing healing with minimal pain, discomfort, and scarring; that is important, especially in specific conditions like cosmetic surgery and vital areas injuries like the chest, joints, and eyes where wounds interfere with vital processes, in addition to specific diseases and disorders such as diabetes, mal-immunity, mal-nutrition, and cancer which prolong the healing. Fifteen adult rabbits were used. After surgical preparation, (2cm) diameter circular full-thickness skin wounds were made on each side of the rabbit's back; the control side was left without treatment, while the treated side was covered with 500 mg of DL-Methionine. The experimental wounds were observed daily to assess wound contraction at 6 hours, 1st,3rd,5th,7th,9th,12th, and 14th days postoperative. Histopathological analysis was performed on wound edge tissue on the 1st, 3rd, 5th, 7th, and 14th postoperative days. The total healing time for both treated and control wounds was measured. DL-Methionine was effective in hemostasis and accelerated the healing process according to the wound contraction assessment and histopathology due to faster maturation of granulation tissue and epithelial cell proliferation compared to the control group. The fibroblast invasion and increased collagen accumulation in the wound marked the transition from inflammation to proliferation.

Keywords

• methionine
• wound healing
• wound powder
• wound contraction
• rabbits