Medium optimization to analyze the protein composition of Bacillus pumilus HR10 antagonizing Sphaeropsis sapinea

Yun Dai; Ya-Hui Wang; Min Li; Mei-Ling Zhu; Tong-Yue Wen; Xiao-Qin Wu

doi:10.1186/s13568-022-01401-1

AMB Express (May 2022)

Medium optimization to analyze the protein composition of Bacillus pumilus HR10 antagonizing Sphaeropsis sapinea

Yun Dai,
Ya-Hui Wang,
Min Li,
Mei-Ling Zhu,
Tong-Yue Wen,
Xiao-Qin Wu

Affiliations

Yun Dai: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University
Ya-Hui Wang: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University
Min Li: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University
Mei-Ling Zhu: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University
Tong-Yue Wen: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University
Xiao-Qin Wu: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University

DOI: https://doi.org/10.1186/s13568-022-01401-1
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

Read online

Keypoints Fermentation medium with high antagonistic activity of HR10 strain was determined. Antagonistic crude protein of HR10 strain purified by Ion-Exchange Chromatography. Composition of the antagonist protein of HR10 strain was analyzed using LC-MS-MS.

Published in AMB Express

ISSN: 2191-0855 (Online)
Publisher: SpringerOpen
Country of publisher: United Kingdom
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Microbiology
Website: http://www.amb-express.com/

About the journal

Abstract

Keywords