Nature Communications (Aug 2021)
CTCF is a barrier for 2C-like reprogramming
- Teresa Olbrich,
- Maria Vega-Sendino,
- Desiree Tillo,
- Wei Wu,
- Nicholas Zolnerowich,
- Raphael Pavani,
- Andy D. Tran,
- Catherine N. Domingo,
- Mariajose Franco,
- Marta Markiewicz-Potoczny,
- Gianluca Pegoraro,
- Peter C. FitzGerald,
- Michael J. Kruhlak,
- Eros Lazzerini-Denchi,
- Elphege P. Nora,
- André Nussenzweig,
- Sergio Ruiz
Affiliations
- Teresa Olbrich
- Laboratory of Genome Integrity, CCR, NCI, NIH
- Maria Vega-Sendino
- Laboratory of Genome Integrity, CCR, NCI, NIH
- Desiree Tillo
- Genetics Branch, CCR, NCI, NIH
- Wei Wu
- Laboratory of Genome Integrity, CCR, NCI, NIH
- Nicholas Zolnerowich
- Laboratory of Genome Integrity, CCR, NCI, NIH
- Raphael Pavani
- Laboratory of Genome Integrity, CCR, NCI, NIH
- Andy D. Tran
- Laboratory of Cancer Biology and Genetics, CCR, NCI, NIH
- Catherine N. Domingo
- Laboratory of Genome Integrity, CCR, NCI, NIH
- Mariajose Franco
- Laboratory of Genome Integrity, CCR, NCI, NIH
- Marta Markiewicz-Potoczny
- Laboratory of Genome Integrity, CCR, NCI, NIH
- Gianluca Pegoraro
- Laboratory of Receptor Biology and Gene Expression, CCR, NCI, NIH
- Peter C. FitzGerald
- Genome Analysis Unit, CCR, NCI, NIH
- Michael J. Kruhlak
- Laboratory of Cancer Biology and Genetics, CCR, NCI, NIH
- Eros Lazzerini-Denchi
- Laboratory of Genome Integrity, CCR, NCI, NIH
- Elphege P. Nora
- Cardiovascular Research Institute, University of California San Francisco
- André Nussenzweig
- Laboratory of Genome Integrity, CCR, NCI, NIH
- Sergio Ruiz
- Laboratory of Genome Integrity, CCR, NCI, NIH
- DOI
- https://doi.org/10.1038/s41467-021-25072-x
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 12
Abstract
Embryos at the 2-cell (2C) stage are totipotent, and overexpression of Dux transcription factor convert embryonic stem cells (ESCs) to a 2C-like state. Here the authors show that DUX-mediated 2C-like reprogramming is associated with DNA damage at CTCF sites and CTCF depletion promotes 2Clike conversion.
