Journal of Extracellular Biology (Oct 2022)
Large‐scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop
- Lucia Paolini,
- Marta Monguió‐Tortajada,
- Marta Costa,
- Fabio Antenucci,
- Mario Barilani,
- Marta Clos‐Sansalvador,
- André Cronemberger Andrade,
- Tom A. P. Driedonks,
- Sara Giancaterino,
- Stephanie M. Kronstadt,
- Rachel R. Mizenko,
- Muhammad Nawaz,
- Xabier Osteikoetxea,
- Carla Pereira,
- Surya Shrivastava,
- Anders Toftegaard Boysen,
- Simonides Immanuel van deWakker,
- Martijn J. C. vanHerwijnen,
- Xiaoqin Wang,
- Dionysios C. Watson,
- Mario Gimona,
- Maria Kaparakis‐Liaskos,
- Konstantin Konstantinov,
- Sai Kiang Lim,
- Nicole Meisner‐Kober,
- Michiel Stork,
- Peter Nejsum,
- Annalisa Radeghieri,
- Eva Rohde,
- Nicolas Touzet,
- Marca H. M. Wauben,
- Kenneth W. Witwer,
- Antonella Bongiovanni,
- Paolo Bergese
Affiliations
- Lucia Paolini
- Department of Molecular and Translational Medicine University of Brescia Brescia Italy
- Marta Monguió‐Tortajada
- CREC Research Program Health Science Research Institute Germans Trias i Pujol (IGTP) Badalona Spain
- Marta Costa
- iBET Instituto de Biologia Experimental e Tecnológica Oeiras Portugal
- Fabio Antenucci
- Department of Veterinary and Animal Sciences University of Copenhagen Copenhagen Denmark
- Mario Barilani
- Laboratory of Regenerative Medicine ‒ Cell Factory Department of Transfusion Medicine and Hematology Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano Italy
- Marta Clos‐Sansalvador
- REMAR‐IGTP Group Germans Trias i Pujol Research Institute (IGTP) & Nephrology Department University Hospital Germans Trias i Pujol (HUGTiP) Badalona (Barcelona) Catalonia Spain
- André Cronemberger Andrade
- Experimental and Clinical Cell Therapy Institute (ExCT) Spinal Cord Injury and Tissue Regeneration Center Paracelsus Medical University Salzburg Austria
- Tom A. P. Driedonks
- CDL Research University Medical Center Utrecht Utrecht University Utrecht The Netherlands
- Sara Giancaterino
- Department of Civil Chemical Environmental and Materials Engineering Alma Mater Studiorum University of Bologna Bologna Italy
- Stephanie M. Kronstadt
- Fischell Department of Bioengineering University of Maryland College Park Maryland USA
- Rachel R. Mizenko
- Department of Biomedical Engineering University of California Davis California USA
- Muhammad Nawaz
- Department of Rheumatology and Inflammation Research Institute of Medicine Sahlgrenska Academy University of Gothenburg Gothenburg Sweden
- Xabier Osteikoetxea
- HCEMM‐SU Extracellular Vesicles Research Group Budapest Hungary
- Carla Pereira
- Exogenus Therapeutics S.A. Cantanhede Portugal
- Surya Shrivastava
- Center for Gene Therapy City of Hope Beckman Research Institute and Hematological Malignancy and Stem Cell Transplantation Institute at the City of Hope Duarte California USA
- Anders Toftegaard Boysen
- Department of Clinical Medicine Aarhus University Aarhus Denmark
- Simonides Immanuel van deWakker
- Department of Cardiology Experimental Cardiology Laboratory University Medical Center Utrecht Utrecht University Utrecht The Netherlands
- Martijn J. C. vanHerwijnen
- Department of Biomolecular Health Sciences Faculty of Veterinary Medicine Utrecht University Utrecht Netherlands
- Xiaoqin Wang
- Discovery Biology Discovery Sciences BioPharmaceuticals R&D AstraZeneca Gothenburg Sweden
- Dionysios C. Watson
- University Hospitals Cleveland Medical Center Cleveland Ohio USA
- Mario Gimona
- GMP Unit Spinal Cord Injury & Tissue Regeneration Centre Salzburg (SCI‐TReCS) Paracelsus Medical University Salzburg Austria
- Maria Kaparakis‐Liaskos
- Department of Microbiology, Anatomy, Physiology and Pharmacology La Trobe University Melbourne Victoria Australia
- Konstantin Konstantinov
- Codiak BioSciences Cambridge Massachusetts USA
- Sai Kiang Lim
- Exosome and Secreted Nano‐vesicle Institute of Molecular & Cell Biology Singapore Singapore
- Nicole Meisner‐Kober
- Department of Biosciences and Medical Biology Paris‐Lodron University Salzburg Hellbrunnerstrasse 34 Salzburg Austria
- Michiel Stork
- Intravacc Bilthoven The Netherlands
- Peter Nejsum
- Department of Clinical Medicine Aarhus University Aarhus Denmark
- Annalisa Radeghieri
- Department of Molecular and Translational Medicine University of Brescia Brescia Italy
- Eva Rohde
- GMP Unit Spinal Cord Injury & Tissue Regeneration Centre Salzburg (SCI‐TReCS) Paracelsus Medical University Salzburg Austria
- Nicolas Touzet
- Centre for Environmental Research Innovation and Sustainability Institute of Technology Sligo Sligo Ireland
- Marca H. M. Wauben
- Department of Biomolecular Health Sciences Faculty of Veterinary Medicine Utrecht University Utrecht Netherlands
- Kenneth W. Witwer
- Department of Molecular and Comparative Pathology Johns Hopkins School of Medicine Baltimore Maryland USA
- Antonella Bongiovanni
- Cell‐Tech HUB and Institute for Research and Biomedical Innovation National Research Council of Italy (CNR) Palermo Italy
- Paolo Bergese
- Department of Molecular and Translational Medicine University of Brescia Brescia Italy
- DOI
- https://doi.org/10.1002/jex2.63
- Journal volume & issue
-
Vol. 1,
no. 10
pp. n/a – n/a
Abstract
Abstract Extracellular vesicles (EVs) large‐scale production is a crucial point for the translation of EVs from discovery to application of EV‐based products. In October 2021, the International Society for Extracellular Vesicles (ISEV), along with support by the FET‐OPEN projects, “The Extracellular Vesicle Foundry” (evFOUNDRY) and “Extracellular vesicles from a natural source for tailor‐made nanomaterials” (VES4US), organized a workshop entitled “massivEVs” to discuss the potential challenges for translation of EV‐based products. This report gives an overview of the topics discussed during “massivEVs”, the most important points raised, and the points of consensus reached after discussion among academia and industry representatives. Overall, the review of the existing EV manufacturing, upscaling challenges and directions for their resolution highlighted in the workshop painted an optimistic future for the expanding EV field.
Keywords
- EV‐based products
- extracellular vesicles
- ISEV workshop
- large‐scale production
- manufacturing
- processing
