Polyamine Metabolites as Biomarkers in Head and Neck Cancer Biofluids

Brian C. DeFelice; Oliver Fiehn; Peter Belafsky; Constanze Ditterich; Michael Moore; Marianne Abouyared; Angela M. Beliveau; D. Gregory Farwell; Arnaud F. Bewley; Shannon M. Clayton; Joehleen A. Archard; Jordan Pavlic; Shyam Rao; Maggie Kuhn; Peter Deng; Julian Halmai; Kyle D. Fink; Andrew C. Birkeland; Johnathon D. Anderson

doi:10.3390/diagnostics12040797

Diagnostics (Mar 2022)

Polyamine Metabolites as Biomarkers in Head and Neck Cancer Biofluids

Brian C. DeFelice,
Oliver Fiehn,
Peter Belafsky,
Constanze Ditterich,
Michael Moore,
Marianne Abouyared,
Angela M. Beliveau,
D. Gregory Farwell,
Arnaud F. Bewley,
Shannon M. Clayton,
Joehleen A. Archard,
Jordan Pavlic,
Shyam Rao,
Maggie Kuhn,
Peter Deng,
Julian Halmai,
Kyle D. Fink,
Andrew C. Birkeland,
Johnathon D. Anderson

Affiliations

Brian C. DeFelice: West Coast Metabolomics Center, University of California, Davis, CA 95616, USA
Oliver Fiehn: West Coast Metabolomics Center, University of California, Davis, CA 95616, USA
Peter Belafsky: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA
Constanze Ditterich: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA
Michael Moore: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA
Marianne Abouyared: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA
Angela M. Beliveau: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA
D. Gregory Farwell: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA
Arnaud F. Bewley: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA
Shannon M. Clayton: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA
Joehleen A. Archard: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA
Jordan Pavlic: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA
Shyam Rao: Department of Radiation Oncology, University of California, Davis, CA 95616, USA
Maggie Kuhn: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA
Peter Deng: Department of Neurology, University of California, Davis, CA 95616, USA
Julian Halmai: Department of Neurology, University of California, Davis, CA 95616, USA
Kyle D. Fink: Department of Neurology, University of California, Davis, CA 95616, USA
Andrew C. Birkeland: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA
Johnathon D. Anderson: Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, CA 95616, USA

DOI: https://doi.org/10.3390/diagnostics12040797
Journal volume & issue: Vol. 12, no. 4
p. 797

Abstract

Read online

Background: Novel, non-invasive diagnostic biomarkers that facilitate early intervention in head and neck cancer are urgently needed. Polyamine metabolites have been observed to be elevated in numerous cancer types and correlated with poor prognosis. The aim of this study was to assess the concentration of polyamines in the saliva and urine from head and neck cancer (HNC) patients, compared to healthy controls. Methods: Targeted metabolomic analysis was performed on saliva and urine from 39 HNC patient samples and compared to 89 healthy controls using a quantitative, targeted liquid chromatography mass spectrometry approach. Results: The metabolites N1-acetylspermine (ASP), N8-acetylspermidine (ASD) and N1,N12-diacetylspermine (DAS) were detected at significantly different concentrations in the urine of HNC patients as compared to healthy controls. Only ASP was detected at elevated levels in HNC saliva as compared to healthy controls. Conclusion: These data suggest that assessment of polyamine-based metabolite biomarkers within the saliva and urine warrants further investigation as a potential diagnostic in HNC patients.

Published in Diagnostics

ISSN: 2075-4418 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Medicine (General)
Website: http://www.mdpi.com/journal/diagnostics

About the journal

Abstract

Keywords