Frontiers in Immunology (May 2017)

Advances in the Isolation of Specific Monoclonal Rabbit Antibodies

  • Zaibao Zhang,
  • Zaibao Zhang,
  • Huijuan Liu,
  • Huijuan Liu,
  • Qian Guan,
  • Lei Wang,
  • Lei Wang,
  • Hongyu Yuan,
  • Hongyu Yuan

DOI
https://doi.org/10.3389/fimmu.2017.00494
Journal volume & issue
Vol. 8

Abstract

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The rabbit monoclonal antibodies (mAbs) have advantages in pharmaceuticals and diagnostics with high affinity and specificity. During the past decade, many techniques have been developed for isolating rabbit mAbs, including single B cell antibody technologies. This review describes the basic characterization of rabbit antibody repertoire and summarizes methods of hybridoma technologies, phage display platform, and single B cell antibody technologies. With advances in antibody function and repertoire analysis, rabbit mAbs will be widely used in therapeutic applications in the coming years.

Keywords