A novel synthesis of stabilized molecularly imprinted polymer for electropolymerization to detect bismethoxycurcumin from curcuminoid

Abstract

The purpose of this study is to synthesize a molecularly imprinted polymer (MIP) for use in an electropolymerization approach to separate bisdemethoxycurcumin (BDMC) from curcuminoid. The template molecule used is 3-(4-hydroxyphenyl) propionic acid (HPPA) serving as a water soluble template with a similar molecular structure to BDMC. Pyrrole is used as the functional monomer (FM). The synthesized ultrathin polymer was reproducible and homogeneous, prepared with a gold electrode. An optimization of conditions to remove the template from the ultrathin film was carried out. The analyte rebinding was investigated with Quartz Crystal Microbalance. Fourier Transform Infra-Red characterized the functional groups before and after washing out the template. An electrodeposition of polymers was achieved at -400 Hz of thickness in 1000 s. The rebinding study showed that adsorption capacity of MIP was higher than that of NIP. Regarding selectivity the BDMC had a higher binding affinity to the MIP than to Curcumin (CUR). MIP sensor demonstrated the ability to discriminate target analyte against a very close analog, curcumin, and exhibited good recovery for use in herbal medicine.

Keywords

• bisdemethoxycurcumin
• electrosynthesis
• functional monomer
• molecularly imprinted polymer
• quartz crystal microbalance