Generation of a RRAGA knockout human iPSC line GIBHi002-A-5 using CRISPR/Cas9 technology

Abstract

Ras-related GTP-binding protein A (RagA), encoded by RRAGA gene, initially senses the availability of cellular amino acids (e.g., leucine) and controls the translocation of mTORC1 to the lysosomal membrane. RagA overexpression appears to be associated with the onset of depression. To discover the biological roles of RagA, we employed the CRISPR/Cas9 system to generate a RRAGA gene knockout stem cell line from human induced pluripotent stem cell (iPSC) iPSN0003. Such RRAGA knockout iPSC cell line may help the development of new therapeutics for depression.