Frontiers in Immunology (Nov 2019)

Multi-Parameter Analysis of Biobanked Human Bone Marrow Stromal Cells Shows Little Influence for Donor Age and Mild Comorbidities on Phenotypic and Functional Properties

  • Anastazja Andrzejewska,
  • Anastazja Andrzejewska,
  • Anastazja Andrzejewska,
  • Rusan Catar,
  • Rusan Catar,
  • Janosch Schoon,
  • Janosch Schoon,
  • Janosch Schoon,
  • Taimoor Hasan Qazi,
  • Taimoor Hasan Qazi,
  • Taimoor Hasan Qazi,
  • Frauke Andrea Sass,
  • Frauke Andrea Sass,
  • Frauke Andrea Sass,
  • Dorit Jacobi,
  • Dorit Jacobi,
  • Antje Blankenstein,
  • Antje Blankenstein,
  • Simon Reinke,
  • Simon Reinke,
  • David Krüger,
  • Mathias Streitz,
  • Mathias Streitz,
  • Stephan Schlickeiser,
  • Stephan Schlickeiser,
  • Sarina Richter,
  • Sarina Richter,
  • Naima Souidi,
  • Naima Souidi,
  • Naima Souidi,
  • Christien Beez,
  • Christien Beez,
  • Christien Beez,
  • Julian Kamhieh-Milz,
  • Ulrike Krüger,
  • Tomasz Zemojtel,
  • Karsten Jürchott,
  • Dirk Strunk,
  • Petra Reinke,
  • Petra Reinke,
  • Petra Reinke,
  • Georg Duda,
  • Georg Duda,
  • Georg Duda,
  • Guido Moll,
  • Guido Moll,
  • Guido Moll,
  • Guido Moll,
  • Sven Geissler,
  • Sven Geissler,
  • Sven Geissler

DOI
https://doi.org/10.3389/fimmu.2019.02474
Journal volume & issue
Vol. 10

Abstract

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Heterogeneous populations of human bone marrow-derived stromal cells (BMSC) are among the most frequently tested cellular therapeutics for treating degenerative and immune disorders, which occur predominantly in the aging population. Currently, it is unclear whether advanced donor age and commonly associated comorbidities affect the properties of ex vivo-expanded BMSCs. Thus, we stratified cells from adult and elderly donors from our biobank (n = 10 and n = 13, mean age 38 and 72 years, respectively) and compared their phenotypic and functional performance, using multiple assays typically employed as minimal criteria for defining multipotent mesenchymal stromal cells (MSCs). We found that BMSCs from both cohorts meet the standard criteria for MSC, exhibiting similar morphology, growth kinetics, gene expression profiles, and pro-angiogenic and immunosuppressive potential and the capacity to differentiate toward adipogenic, chondrogenic, and osteogenic lineages. We found no substantial differences between cells from the adult and elderly cohorts. As positive controls, we studied the impact of in vitro aging and inflammatory cytokine stimulation. Both conditions clearly affected the cellular properties, independent of donor age. We conclude that in vitro aging rather than in vivo donor aging influences BMSC characteristics.

Keywords