Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions

Karl-Uwe Reusswig; Julia Bittmann; Martina Peritore; Mathilde Courtes; Benjamin Pardo; Michael Wierer; Matthias Mann; Boris Pfander

doi:10.1038/s41467-022-34379-2

Nature Communications (Nov 2022)

Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions

Karl-Uwe Reusswig,
Julia Bittmann,
Martina Peritore,
Mathilde Courtes,
Benjamin Pardo,
Michael Wierer,
Matthias Mann,
Boris Pfander

Affiliations

Karl-Uwe Reusswig: DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry
Julia Bittmann: DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry
Martina Peritore: DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry
Mathilde Courtes: Institut de Génétique Humaine (IGH), Université de Montpellier – Centre National de la Recherche Scientifique
Benjamin Pardo: Institut de Génétique Humaine (IGH), Université de Montpellier – Centre National de la Recherche Scientifique
Michael Wierer: Proteomics and Signal Transduction, Max Planck Institute of Biochemistry
Matthias Mann: Proteomics and Signal Transduction, Max Planck Institute of Biochemistry
Boris Pfander: DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry

DOI: https://doi.org/10.1038/s41467-022-34379-2
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 20

Abstract

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Reusswig et al. use engineered systems to force DNA replication in the G1 phase of the cell cycle. This unscheduled G1 replication shows hallmarks of S phase replication, but leads to over-replication and DNA breaks from replication collisions.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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