Química Nova (Jan 2014)

LC-MS/MS method applied to preclinical pharmacokinetic investigation of olanzapine-loaded lipid-core nanocapsules

  • Frantiescoli A. Dimer,
  • Maiara C. Pigatto,
  • Adriana R. Pohlmann,
  • Teresa Dalla Costa,
  • Silvia S. Guterres

DOI
https://doi.org/10.5935/0100-4042.20140195
Journal volume & issue
Vol. 37, no. 8
pp. 1371 – 1376

Abstract

Read online

In spite of different methods reported in the literature to determine olanzapine in biological fluids, all of them used high volumes of plasma. Therefore, the purpose of this paper was to develop an LC-MS/MS method using small plasma volume (0.1 mL) to apply in a preclinical pharmacokinetic investigation. The method was linear over the concentration ranges of 10 - 1000 ng mL-1. Extraction recoveries, stability, and validation parameters were evaluated. Results were within the acceptable limits of international guidelines. A significant decrease in clearance led to a significant 2.26-times increase in AUC0 - 6h of olanzapine-loaded lipid-core nanocapsules compared with free-olanzapine.

Keywords