Uc.416 + A promotes epithelial-to-mesenchymal transition through miR-153 in renal cell carcinoma

Yohei Sekino; Naoya Sakamoto; Keisuke Goto; Ririno Honma; Yoshinori Shigematsu; Thang Pham Quoc; Kazuhiro Sentani; Naohide Oue; Jun Teishima; Fumi Kawakami; Jose A Karam; Kanishka Sircar; Akio Matsubara; Wataru Yasui

doi:10.1186/s12885-018-4863-y

BMC Cancer (Oct 2018)

Uc.416 + A promotes epithelial-to-mesenchymal transition through miR-153 in renal cell carcinoma

Yohei Sekino,
Naoya Sakamoto,
Keisuke Goto,
Ririno Honma,
Yoshinori Shigematsu,
Thang Pham Quoc,
Kazuhiro Sentani,
Naohide Oue,
Jun Teishima,
Fumi Kawakami,
Jose A Karam,
Kanishka Sircar,
Akio Matsubara,
Wataru Yasui

Affiliations

Yohei Sekino: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences
Naoya Sakamoto: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences
Keisuke Goto: Cancer Biology Program, University of Hawaii Cancer Center
Ririno Honma: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences
Yoshinori Shigematsu: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences
Thang Pham Quoc: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences
Kazuhiro Sentani: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences
Naohide Oue: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences
Jun Teishima: Department of Urology, Hiroshima University Institute of Biomedical and Health Sciences
Fumi Kawakami: Departments of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center
Jose A Karam: Departments of Urology, The University of Texas MD Anderson Cancer Center
Kanishka Sircar: Departments of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center
Akio Matsubara: Department of Urology, Hiroshima University Institute of Biomedical and Health Sciences
Wataru Yasui: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences

DOI: https://doi.org/10.1186/s12885-018-4863-y
Journal volume & issue: Vol. 18, no. 1
pp. 1 – 10

Abstract

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Abstract Background The transcribed ultraconserved regions (T-UCRs) are a novel class of non-coding RNAs that are absolutely conserved across species and are involved in carcinogenesis in some cancers. However, the expression and biological role of T-UCRs in renal cell carcinoma (RCC) remain poorly understood. This study aimed to examine the expression and functional role of Uc.416 + A and analyze the association between Uc.416 + A and epithelial-to-mesenchymal transition in RCC. Methods Expression of Uc.416 + A in 35 RCC tissues, corresponding normal kidney tissues and 13 types of normal tissue samples was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). We performed a cell growth and migration assay in RCC cell line 786-O transfected with negative control and siRNA for Uc.416 + A. We evaluated the relation between Uc.416 + A and miR-153, which has a complimentary site of Uc.416 + A. Results qRT-PCR analysis revealed that the expression of Uc.416 + A was higher in RCC tissues than that in corresponding normal kidney tissues. Inhibition of Uc.416 + A reduced cell growth and cell migration activity. There was an inverse correlation between Uc.416 + A and miR-153. Western blot analysis showed Uc.416 + A modulated E-cadherin, vimentin and snail. The expression of Uc.416 + A was positively associated with the expression of SNAI1, VIM and inversely associated with the expression of CDH1. Conclusions The expression of Uc.416 + A was upregulated in RCC and especially in RCC tissues with sarcomatoid change. Uc.416 + A promoted epithelial-to-mesenchymal transition through miR-153. These results suggest that Uc.416 + A may be a promising therapeutic target.

Published in BMC Cancer

ISSN: 1471-2407 (Online)
Publisher: BMC
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: http://bmccancer.biomedcentral.com

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