Frontiers in Veterinary Science (Nov 2020)
Comparison of Pathogenicity of Different Infectious Doses of H3N2 Canine Influenza Virus in Dogs
- Yongbo Liu,
- Yongbo Liu,
- Yongbo Liu,
- Cheng Fu,
- Gang Lu,
- Gang Lu,
- Gang Lu,
- Jie Luo,
- Jie Luo,
- Jie Luo,
- Shaotang Ye,
- Shaotang Ye,
- Shaotang Ye,
- Jiajun Ou,
- Jiajun Ou,
- Jiajun Ou,
- Xiangbin Wang,
- Xiangbin Wang,
- Xiangbin Wang,
- Haibin Xu,
- Haibin Xu,
- Haibin Xu,
- Ji Huang,
- Ji Huang,
- Ji Huang,
- Liyan Wu,
- Liyan Wu,
- Liyan Wu,
- Xin Zhang,
- Xin Zhang,
- Xin Zhang,
- Peixin Wu,
- Peixin Wu,
- Peixin Wu,
- Shoujun Li,
- Shoujun Li,
- Shoujun Li
Affiliations
- Yongbo Liu
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yongbo Liu
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Yongbo Liu
- Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, China
- Cheng Fu
- Institute of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China
- Gang Lu
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Gang Lu
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Gang Lu
- Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, China
- Jie Luo
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Jie Luo
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Jie Luo
- Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, China
- Shaotang Ye
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Shaotang Ye
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Shaotang Ye
- Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, China
- Jiajun Ou
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Jiajun Ou
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Jiajun Ou
- Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, China
- Xiangbin Wang
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Xiangbin Wang
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Xiangbin Wang
- Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, China
- Haibin Xu
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Haibin Xu
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Haibin Xu
- Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, China
- Ji Huang
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Ji Huang
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Ji Huang
- Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, China
- Liyan Wu
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Liyan Wu
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Liyan Wu
- Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, China
- Xin Zhang
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Xin Zhang
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Xin Zhang
- Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, China
- Peixin Wu
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Peixin Wu
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Peixin Wu
- Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, China
- Shoujun Li
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Shoujun Li
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Shoujun Li
- Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, China
- DOI
- https://doi.org/10.3389/fvets.2020.580301
- Journal volume & issue
-
Vol. 7
Abstract
The canine influenza virus (CIV) outbreaks have raised concerns as they pose a threat to the health of dogs. The successful construction of a canine influenza (CI) infection model is essential to study the CIV. Here we investigated the pathogenicity of different infectious doses of H3N2 CIV in Beagle dogs. Thirty-seven healthy Beagle dogs were used in the experiment and were infected with 103, 104, 105, and 106 50% egg-infectious doses (EID50). Compared to the dogs in the other three groups, those in the 106 EID50 group presented with obvious clinical symptoms, high virus titer, and typical pathological changes. Considering the ensemble of clinical scores, body temperature, virus shedding, lung lesions, pathological section scores, and visceral virus titers, we determined that 106 EID50 is the minimum infectious dose for the Beagle infection model. The other three infectious doses had almost no clinical symptoms. These results indicate that 106 EID50 is the minimum infectious dose of H3N2 CIV that can cause obvious clinical manifestations and pathological changes associated with CI in Beagle dogs. The theoretical framework developed in this research will guide the establishment of an infection model of CIV for future research.
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