Cyclic Tetrapeptides with Synergistic Antifungal Activity from the Fungus <i>Aspergillus westerdijkiae</i> Using LC-MS/MS-Based Molecular Networking

Junjie Han; Hanying Wang; Rui Zhang; Huanqin Dai; Baosong Chen; Tao Wang; Jingzu Sun; Wenzhao Wang; Fuhang Song; Erwei Li; Zhitang Lyu; Hongwei Liu

doi:10.3390/antibiotics11020166

Antibiotics (Jan 2022)

Cyclic Tetrapeptides with Synergistic Antifungal Activity from the Fungus <i>Aspergillus westerdijkiae</i> Using LC-MS/MS-Based Molecular Networking

Junjie Han,
Hanying Wang,
Rui Zhang,
Huanqin Dai,
Baosong Chen,
Tao Wang,
Jingzu Sun,
Wenzhao Wang,
Fuhang Song,
Erwei Li,
Zhitang Lyu,
Hongwei Liu

Affiliations

Junjie Han: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Hanying Wang: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Rui Zhang: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Huanqin Dai: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Baosong Chen: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Tao Wang: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Jingzu Sun: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Wenzhao Wang: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Fuhang Song: School of Light Industry, Beijing Technology and Business University, Beijing 100048, China
Erwei Li: Institutional Center for Shared Technologies and Facilities, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Zhitang Lyu: School of Life Sciences, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China
Hongwei Liu: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

DOI: https://doi.org/10.3390/antibiotics11020166
Journal volume & issue: Vol. 11, no. 2
p. 166

Abstract

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Fungal natural products play a prominent role in the development of pharmaceuticalagents. Two new cyclic tetrapeptides (CTPs), westertide A (1) and B (2), with eight known compounds (3–10) were isolated from the fungus Aspergillus westerdijkiae guided by OSMAC (one strain-many compounds) and molecular networking strategies. The structures of new compounds were unambiguously determined by a combination of NMR and mass data analysis, and chemical methods. All of the isolates were evaluated for antimicrobial effects, synergistic antifungal activity, cytotoxic activity, and HDAC inhibitory activity. Compounds 1–2 showed synergistic antifungal activity against Candida albicans SC5314 with the presence of rapamycin and weak HDAC (histone deacetylase) inhibitory activity. These results indicate that molecular networking is an efficient approach for dereplication and identification of new CTPs. CTPs might be a good starting point for the development of synergistic antifungal agents.

Published in Antibiotics

ISSN: 2079-6382 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.mdpi.com/journal/antibiotics

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