COMPARATIVE-EVALUATION-OF-TWO-VIABILITY ASSAYS ON BIODEGRADABLE POLYMER FOAMS FOR POTENTIAL BONE REGENERATION

Abstract

The purpose of the present study was to evaluate comparatively the cytotoxicity of two foams prepared by phosphorylated cellulose and phosphorylated PVA using two common assays, MTT classic assay and Cell-titer blue assay. Two foams with potential applications for bone substitute were evaluated. Both evaluated samples (phosphorylated cellulose and phosphorylated PVA) conditioned as foams showed a very good compatibility against osteoblast-like cells. The main advantage of the cell titer blue protocol for cell proliferation against MTT assay is represented by the possibility of direct addition of the assay reagents onto the wells with the material to be evaluated.

Keywords

• CellTiter Blue assay
• MTT assay
• polymer foam