Green synthesis of AgNPs@PPE and its Pseudomonas aeruginosa biofilm formation activity compared to pomegranate peel extract

Abstract

Reza Habibipour,1 Leila Moradi-Haghgou,1 Abbas Farmany21Department of Microbiology, Hamedan Branch, Islamic Azad University, Hamedan, Iran; 2Dental Research Center, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, IranCorrespondence: Reza HabibipourDepartment of Microbiology, Islamic Azad University, Hamedan Branch, Prof. Mousivand Blvd., Imam Khomeini Blvd, Hamedan 6518115743, IranEmail [email protected] FarmanyDental Research Center, School of Dentistry, Hamadan University of Medical Sciences, Shahid Fahmideh Street, Hamadan 6517838677, IranEmail [email protected]: Bacteria are able to form biofilm on the biotic and abiotic surfaces which helps to protect themselves from deleterious conditions, predation, desiccation, and exposure to antibacterial substances. About 80% of bacterial infections are caused by those bacteria living in the biofilm. Pseudomonas aeruginosa, a gram-negative, non-fermentative bacillus, and the ubiquitous bacterium is an important opportunistic pathogen notorious for biofilm formation and is remarkably resistant against most antibiotics multiple front-line antibiotics, which significantly contributes to eradication failure. The aim of this paper was to evaluate the anti-biofilm formation activity of Ag@PPEs gainst P. aeruginosa bacteria.Methods: An aqueous extract of black pomegranate peel was used for the synthesis of silver nanoparticles (AgNPs@PPE). The characteristics, anti-biofilm formation and cell toxicity of AgNPs@PPE were examined in vitro.Results: Absorbance at λmax 372 nm which is related to the surface plasmon resonance, confirms the AgNPs@PPE formation. XRD pattern showed the face-centered qubic (fcc) crystalline structure of AgNPs. TEM images showed that spherical AgNPs size is ranged between 32 and 85 nm. The AgNPs@PPE showed inhibition effect against P. aeruginosa biofilm formation at 0.1 to 0.5 mg/ml concentrations. Cell toxicity assay showed that at 400 μg/ml, AgNPs@PPE were safe without a significant toxicity in L929 cell line.Conclusion: These data indicate that co-treatment of PPE and AgNPs@PPE significantly decreased the biofilm formation rate. Furthermore, no significant toxicity of AgNPs@PPE was shown against L929 cell line at 400 μg/ml concentration.Keywords: AgNPs, green synthesis, pomegranate peel extract, antibacterial activity

Keywords

• AgNPs
• green synthesis
• pomegranate peel extract
• antibacterial activity