INTEREST OF BOVINE FOLLICULAR FLUID IN THE ENRICHMENT OF MEDIA FOR THE IN VITRO MATURATION OF BOVINE OOCYTES

Abstract

Our aim is to verify the interest of using bovine follicular fluid as an enrichment of basal media for the in vitro maturation of cow oocytes. The oocytes are collected from slaughterhouse ovaries as well as the follicular fluid used as an additive. The ovaries were transported at room temperature and the follicles aspirated within two hours of slaughter. The maturation was carried out in an oven at 38°C, 5% CO2 and in an atmosphere saturated with humidity. In this work we tested three media with different proportions of bovine follicular fluid (0%, 10% and 50%) then we compared the expansion rates of the cumulus clouds with that obtained with a commercial medium that does not require CO2.The best expansion rates were obtained with the basic medium added with 10% follicular fluid (57.64%), this mixture seems to be optimal because this result has been confirmed by data from the bibliography. The comparison with the commercial culture medium gave almost similar results (57.64% vs 56.16%) which is a positive result in itself despite the absence of statistical significance