Study of milk production genes and their association with production traits in Rathi cattle

Abstract

The study aimed to identify polymorphism of LEP, and STAT5A milk-producing genes and their association with production traits in Rathi cattle. An overall 160 animals were selected from a population of Rathi cattle from Livestock Research Station, Rajasthan University of Veterinary and Animals Sciences, Bikaner (Rajasthan). The phenotypic information on total milk yield, peak yield and lactation length were recorded from the years 2012- 2018. Each animal’s milk sample (100 ml) was analysed for milk composition parameters. Genomic DNA was extracted from the whole blood sample through the spin column method and association analysis was done. The polymorphism have been observed in exon-3 (454 bp) of LEP gene, intron 9-10 (224 bp) and intron-15-exon-16 (379 bp) of STAT5A genes by PCR-SSCP and revealed two types of genotypic pattern. The STAT5A exon-7 (215 bp) also showed polymorphism with three genotypic patterns. The AB pattern of LEP exon-3 and AA pattern of intron 9-10 and intron-15-exon-16 of STAT5A genes showed a significant effect on total milk yield. The BB genotypic pattern of the STAT5A exon-7 gene showed a significant impact on peak yield. The AB pattern of LEP and STAT5A genes were associated with more milk fat % in the studied population of Rathi cattle. All these genes showed a significant effect on total solids. The association analysis of LEP and STAT5A genes with different milk production and milk composition traits illustrated the worth of these genes for marker-assisted selection of dairy cattle. Thus, the present study was designed to unmask the relevant genetic factors responsible for variation in milk performance traits.

Keywords

• Genotype
• Lactation length
• PCR
• Peak yield
• Polymorphism
• SSCP