Catalase Enzyme Gene Expression and Oxidant Markers’ Levels in Trained Women: Effect of Incremental Exercise

Abstract

Introduction: There are few studies regarding the field of catalase gene expression, antioxidant and oxidant response in trained women, thus these markers’ levels have not been designated within Iranian trained women specially in regard to incremental exercise. Therefore, this study aimed to investigate Catalase enzyme Gene expression and oxidant levels in trained women. Methods: A repeated measure design was used for this study. Fourteen trained young women in the age range of 23-25 years from urmia city were volunteered as subjects. Venous blood samples were taken in three stages, before GXT exercise test (graduate exercise test) (speed: 7/5 mile, slope grade: 6%, exercise time: 30 minutes), immediately and 3 hours after exercise. Real time PCR method was used for analysis of the mRNA of Catalase gene expression and the eutoanalyzer method was also applied for measurement of MDA activity. Results: MDA (Malondealdeide) levels increased immediately and 3 h after exercise, though it proved to be statistically significant only in recovery stage (3 h after exercise) (P<0/002). Catalase gene expression significantly increased after exercise, but this difference wasn’t significant in recovery stage (P<0/03). Also, TAS (total antioxidant status) concentration increase was significant after training exercise (P0/065). Conclusion: Incremental exercise test has increased catalase gene expression and antioxidant and free radicals levels in trained women.

Keywords

• Catalase
• Gene expression
• Trained women