Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers

Abstract

Summary: Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis.For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021).

Keywords

• Single Cell
• Developmental biology
• Genetics
• Microscopy
• Model Organisms
• Molecular Biology