Scientific Reports (Jun 2017)

miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p

  • Cristina Gutiérrez-Vázquez,
  • Ana Rodríguez-Galán,
  • Marcos Fernández-Alfara,
  • María Mittelbrunn,
  • Fátima Sánchez-Cabo,
  • Dannys Jorge Martínez-Herrera,
  • Marta Ramírez-Huesca,
  • Alberto Pascual-Montano,
  • Francisco Sánchez-Madrid

DOI
https://doi.org/10.1038/s41598-017-03689-7
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 8

Abstract

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Abstract microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3′UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells.