Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2

Hector Huerga Encabo; Rachel Ulferts; Aneesh Sharma; Rupert Beale; Dominique Bonnet

STAR Protocols (Dec 2021)

Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2

Hector Huerga Encabo,
Rachel Ulferts,
Aneesh Sharma,
Rupert Beale,
Dominique Bonnet

Affiliations

Hector Huerga Encabo: Hematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK; Corresponding author
Rachel Ulferts: Cell Biology of Infection Laboratory, The Francis Crick Institute, London NW1 1AT, UK; Corresponding author
Aneesh Sharma: Hematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK
Rupert Beale: Cell Biology of Infection Laboratory, The Francis Crick Institute, London NW1 1AT, UK
Dominique Bonnet: Hematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK; Corresponding author

Journal volume & issue: Vol. 2, no. 4
p. 100903

Abstract

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Summary: Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay.For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021)

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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