Generation of a human SLC12A3 knock-in human induced pluripotent stem cell line (CMCi014-A-82) using CRISPR-Cas9 system

Sun Woo Lim; Kang In Lee; Sheng Cui; Xianying Fang; Yoo Jin Shin; Hanbi Lee; Chul Woo Yang; Jae Young Lee; Byung Ha Chung

Stem Cell Research (Dec 2024)

Generation of a human SLC12A3 knock-in human induced pluripotent stem cell line (CMCi014-A-82) using CRISPR-Cas9 system

Sun Woo Lim,
Kang In Lee,
Sheng Cui,
Xianying Fang,
Yoo Jin Shin,
Hanbi Lee,
Chul Woo Yang,
Jae Young Lee,
Byung Ha Chung

Affiliations

Sun Woo Lim: Transplant Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea
Kang In Lee: R&D Center, ToolGen, Inc., Seoul, Republic of Korea
Sheng Cui: Transplant Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea
Xianying Fang: Transplant Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea
Yoo Jin Shin: Transplant Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea
Hanbi Lee: Transplant Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Internal Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul, Republic of Korea
Chul Woo Yang: Transplant Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Internal Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul, Republic of Korea
Jae Young Lee: R&D Center, ToolGen, Inc., Seoul, Republic of Korea; Corresponding authors at: R&D Center, ToolGen, Inc., 8F, 172 Magokjungang-ro, Gangseo-gu, Seoul 07789, Republic of Korea (J.Y. Lee). Transplantation Research Centre & Division of Nephrology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, 222 Banpo-daero, Seocho-gu, 06591, Republic of Korea (B.H. Chung).
Byung Ha Chung: Transplant Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Internal Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul, Republic of Korea; Corresponding authors at: R&D Center, ToolGen, Inc., 8F, 172 Magokjungang-ro, Gangseo-gu, Seoul 07789, Republic of Korea (J.Y. Lee). Transplantation Research Centre & Division of Nephrology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, 222 Banpo-daero, Seocho-gu, 06591, Republic of Korea (B.H. Chung).

Journal volume & issue: Vol. 81
p. 103522

Abstract

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Gitelman’s disease is caused by a genetic mutation in the solute carrier family 12 member 3 (SLC12A3) gene, which encodes the sodium chloride cotransporter. In this study, we generated a stable human induced pluripotent stem cell (hiPSC) line, WTC11-SLC12A3 (CMCi014-A-82), by knocking in the entire SLC12A3 gene at the SHS231 locus in healthy wild-type control hiPSCs (WTC11). We verified that WTC11-SLC12A3 expressed pluripotency markers and exhibited normal stem cell morphology. Furthermore, this cell line maintains a normal karyotype and can differentiate into the three germ layers. Therefore, this cell line may provide a basis for gene therapy for Gitelman’s disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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