Nature Communications (Mar 2020)

Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

  • Oliver Xiaoou Dong,
  • Shu Yu,
  • Rashmi Jain,
  • Nan Zhang,
  • Phat Q. Duong,
  • Corinne Butler,
  • Yan Li,
  • Anna Lipzen,
  • Joel A. Martin,
  • Kerrie W. Barry,
  • Jeremy Schmutz,
  • Li Tian,
  • Pamela C. Ronald

DOI
https://doi.org/10.1038/s41467-020-14981-y
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 10

Abstract

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Existing examples of targeted gene insertion in plants either rely on a selectable marker gene or result in short DNA inserts. Here, the authors use an optimized CRISPR-Cas9 method to insert a 5.2 kb carotenoid biosynthesis cassette into genomic safe harbors in rice, and obtain marker-free lines with high carotenoid content.