Clinical feasibility of a label-free SERS assay for therapeutic drug monitoring of methotrexate

Elodie Dumont; Gohar Soufi; Yaman Göksel; Roman Slipets; Raheel Altaf Raja; Kjeld Schmiegelow; Kinga Zor; Anja Boisen

Sensing and Bio-Sensing Research (Jun 2024)

Clinical feasibility of a label-free SERS assay for therapeutic drug monitoring of methotrexate

Elodie Dumont,
Gohar Soufi,
Yaman Göksel,
Roman Slipets,
Raheel Altaf Raja,
Kjeld Schmiegelow,
Kinga Zor,
Anja Boisen

Affiliations

Elodie Dumont: Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Kgs. Lyngby 2800, Denmark; BioInnovation Institute Foundation, Copenhagen N, 2200, Denmark; Corresponding authors: Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Kgs. Lyngby 2800, Denmark.
Gohar Soufi: Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Kgs. Lyngby 2800, Denmark; BioInnovation Institute Foundation, Copenhagen N, 2200, Denmark; Corresponding authors: Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Kgs. Lyngby 2800, Denmark.
Yaman Göksel: Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Kgs. Lyngby 2800, Denmark; BioInnovation Institute Foundation, Copenhagen N, 2200, Denmark
Roman Slipets: Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Kgs. Lyngby 2800, Denmark; BioInnovation Institute Foundation, Copenhagen N, 2200, Denmark
Raheel Altaf Raja: Department of Paediatrics and Adolescent Medicine, Rigshospitalet University Hospital, Copenhagen 2100, Denmark
Kjeld Schmiegelow: Department of Paediatrics and Adolescent Medicine, Rigshospitalet University Hospital, Copenhagen 2100, Denmark
Kinga Zor: Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Kgs. Lyngby 2800, Denmark; BioInnovation Institute Foundation, Copenhagen N, 2200, Denmark
Anja Boisen: Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Kgs. Lyngby 2800, Denmark; BioInnovation Institute Foundation, Copenhagen N, 2200, Denmark

Journal volume & issue: Vol. 44
p. 100649

Abstract

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We present a therapeutic drug monitoring (TDM) feasibility study using a label-free surface-enhanced Raman spectroscopy (SERS)-based assay for quantifying methotrexate (MTX) from samples collected from children with acute lymphoblastic leukemia undergoing high-dose MTX therapy. We show that, when combined with an appropriate sample preparation (solid phase extraction) and multivariate data analysis (partial least squares regression), the SERS assay demonstrated a good correlation with a reference chromatographic method (r = 0.889, p < 0.005). We also found that the SERS-based approach underestimated MTX concentration, with a 10% bias calculated, but both methods showed similar variability (RSD of 8.4% for HPLC and 11.6% for SERS). The presented results are strong evidence that SERS can be used for TDM of MTX, and this work brings us one step closer to implementing a SERS-based assay in clinics.

Published in Sensing and Bio-Sensing Research

ISSN: 2214-1804 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Engineering (General). Civil engineering (General)
Website: http://www.journals.elsevier.com/sensing-and-bio-sensing-research/

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