Chemo-preventive Activity of Ethanolic Extracts of Newbouldia laevis and Olax subscorpioidea Leaves on Methylnitrosourea–induced Stroma Fibrosis in Breast Tissues of Female Albino Rats

Abstract

Breast cancer represents the most common neoplastic disease in females, accounting for up to one third of new diagnoses of women’s cancer in certain regions of the world. The chemo-preventive activity of ethanolic extracts of Newbouldia laevis and Olax subscorpioidea leaves on methylnitrosourea (MNU)–induced stroma fibrosis in female albino rats was evaluated. Quantitative and qualitative phytochemical screening was carried out to identify and determine the quantity of bioactive compounds. Haematoxylin and Eosin staining procedure was used to assess breast tissues of experimental animals. Cancer antigen (CA) markers 15-3, 27-29 and carcinoembryonic antigen (CEA) in the blood of experimental animals were evaluated using an automated procedure. Quantitative phytochemical screening showed that Olax subscorpioidea and Newbouldia laevis contained alkaloid in the highest amount. Histopathological assay revealed the presence of stromal fibrosis in the breast tissues of 8 out of 10 rats administered with NMU. Levels of CA 15-3, CA 27-29, CEA were significantly (p < 0.05) elevated in MNU administered group in comparison to the negative control. Treatment with Olax subscorpioidea leave extract significantly (p < 0.05) ameliorated CA 15-3, CA 27-29 and CEA levels. However, treatment with Newbouldia laevis leave extract significantly (p < 0.05) ameliorated CA 15-3 and CEA levels only. Findings from this study showed that ethanolic extracts of Olax subscorpioidea and Newbouldia laevis leaves have chemo-preventive activity in NMU-induced stroma fibrosis in the breast tissue of female albino rats. However more studies on the discovery and validation of bioactive components of the plants are needed.

Keywords

• Methylnitrosourea;
• Olax subscorpioidea;
• Newbouldia laevis;
• Breast cancer;
• Stroma fibrosis