Evaluation of Scopoletin from Penicillium janthinellum Snef1650 for the Control of Heterodera glycines in Soybean

Jichen Yan; Zhifu Xing; Piao Lei; Aatika Sikandar; Ruowei Yang; Yuanyuan Wang; Xiaofeng Zhu; Xiaoyu Liu; Haiyan Fan; Yuanhu Xuan; Lijie Chen; Yuxi Duan

doi:10.3390/life11111143

Life (Oct 2021)

Evaluation of Scopoletin from Penicillium janthinellum Snef1650 for the Control of Heterodera glycines in Soybean

Jichen Yan,
Zhifu Xing,
Piao Lei,
Aatika Sikandar,
Ruowei Yang,
Yuanyuan Wang,
Xiaofeng Zhu,
Xiaoyu Liu,
Haiyan Fan,
Yuanhu Xuan,
Lijie Chen,
Yuxi Duan

Affiliations

Jichen Yan: Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China
Zhifu Xing: Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China
Piao Lei: Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China
Aatika Sikandar: Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China
Ruowei Yang: Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China
Yuanyuan Wang: Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China
Xiaofeng Zhu: Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China
Xiaoyu Liu: Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China
Haiyan Fan: Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China
Yuanhu Xuan: Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China
Lijie Chen: Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China
Yuxi Duan: Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China

DOI: https://doi.org/10.3390/life11111143
Journal volume & issue: Vol. 11, no. 11
p. 1143

Abstract

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Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is responsible for causing a major soybean disease globally. The fungal strain Penicillium janthinellum Snef1650 was evaluated against H. glycines. However, the effective determinants of the P. janthinellum strain are unknown. By performing pot experiments, a functioning compound was isolated from P. janthinellum Snef1650 through organic solvent extraction, semi-preparative HPLC, Sephadex LH-20 column chromatography, and silica gel column chromatography, and the isolated compound was identified to be scopoletin through 1H NMR, 13C NMR, and HPLC–MS. The pot experiments indicated that the treatment of soybean seeds with scopoletin drastically reduced the SCN population. The field experiments performed in 2017 and 2018 revealed that scopoletin decreased over 43.7% juveniles in the roots and over 61.55% cysts in the soil. Scopoletin treatment also promoted soybean growth and improved its yield, with an increase in plot yield by >5.33%. Scopoletin obtained from P. janthinellum Snef1650 could be used as an anti-H. glycines biocontrol agent.

Published in Life

ISSN: 2075-1729 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science
Website: http://www.mdpi.com/journal/life

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