Membranes (Dec 2023)

Evaluation of the Microbial Profile on the Polydioxanone Membrane and the Collagen Membrane Exposed to Multi-Species Subgingival Biofilm: An In Vitro Study

  • Marcus Vinícius Cintra Moreira,
  • Luciene C. Figueiredo,
  • Marcelo Augusto Ruiz da Cunha Melo,
  • Fabio Hideaki Uyeda,
  • Lucas Daylor Aguiar da Silva,
  • Tatiane Tiemi Macedo,
  • Roberto Sacco,
  • Carlos Fernando Mourão,
  • Jamil A. Shibli,
  • Bruno Bueno-Silva

DOI
https://doi.org/10.3390/membranes13120907
Journal volume & issue
Vol. 13, no. 12
p. 907

Abstract

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Dehiscence in surgeries involving membranes often leads to bacterial contamination, hindering the healing process. This study assessed bacterial colonization on various membrane materials. Polydioxanone (PDO) membranes, with thicknesses of 0.5 mm and 1 mm, and a collagen membrane were examined. Packages containing polystyrene pins were crafted using these membranes, attached to 24-well plates, and exposed to oral bacteria from supra and subgingival biofilm. After a week’s anaerobic incubation, biofilm formation was evaluated using the DNA–DNA hybridization test. Statistical analysis employed the Kruskal–Wallis test with Dunn’s post hoc test. The biofilm on the polystyrene pins covered by the 0.5 mm PDO membrane showed a higher count of certain pathogens. The collagen membrane had a greater total biofilm count on its inner surface compared to both PDO membranes. The external collagen membrane face had a higher total biofilm count than the 0.5 mm PDO membrane. Furthermore, the 1 mm PDO membrane exhibited a greater count of specific pathogens than its 0.5 mm counterpart. In conclusion, the collagen membrane presented more biofilm and pathogens both internally and on its inner surface.

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