Evaluation of the qualitative and quantitative composition of or al microbiota in patients with medial osteonecrosis of the jaw

Abstract

Aim. To determine the qualitative and quantitative composition of the oral microbiota in patients with medial osteonecrosis of the jaw (MOJ). Materials and methods. Microbiota was evaluated in 15 patients with medial osteonecrosis of the jaw. An assessment was made of 12 groups of optional and obligate-anaerobic microorganisms. Evaluation of the qualitative and quantitative composition of oral microbiota in patients with MOJ was performed by real-time polymerase chain reaction (PCR-RT). Results. The aerobic-anaerobic microbiota composition in patients with MOJ in the oral fluid and separated from the fistula is presented. The values of the total bacterial mass (TBM) and all anaerobic microorganisms in the oral fluid in patients with MOJ before treatment were higher by 1-3 orders of magnitude than in healthy individuals. In the jaw, separated from the fistula in the zone of necrosis of the jaw, these parameters were higher by 2-4 orders than in the oral fluid. Prior to surgery, a course of antibiotic therapy was conducted for 7 days. As a result of the therapy, the parameters of anaerobic microflora in the oral fluid decreased. The use of cefazolin was more effective than lincomycin. In 1 year after surgical treatment, the index of TBM and the indices of anaerobic microorganisms did not differ from those of healthy individuals. Conclusion. Patients with medical osteonecrosis of the jaw have a high level of anaerobic pathogens in the oral microbiota. The course of antibiotic therapy and surgical intervention are effective methods of treatment of MOJ, as they lead to effective sanation, and allow further endoprosthetics.

Keywords

• medical osteonecrosis of the jaw
• conditionally pathogenic microorganisms of the oral cavity
• pcr-rt
• surgical treatment