Nucleotide resolution mapping of influenza A virus nucleoprotein-RNA interactions reveals RNA features required for replication

Graham D. Williams; Dana Townsend; Kristine M. Wylie; Preston J. Kim; Gaya K. Amarasinghe; Sebla B. Kutluay; Adrianus C. M. Boon

doi:10.1038/s41467-018-02886-w

Nature Communications (Jan 2018)

Nucleotide resolution mapping of influenza A virus nucleoprotein-RNA interactions reveals RNA features required for replication

Graham D. Williams,
Dana Townsend,
Kristine M. Wylie,
Preston J. Kim,
Gaya K. Amarasinghe,
Sebla B. Kutluay,
Adrianus C. M. Boon

Affiliations

Graham D. Williams: Department of Medicine at Washington University School of Medicine
Dana Townsend: Department of Molecular Microbiology at Washington University School of Medicine
Kristine M. Wylie: Department of Pediatrics at Washington University School of Medicine
Preston J. Kim: Department of Pathology and Immunology at Washington University School of Medicine
Gaya K. Amarasinghe: Department of Pathology and Immunology at Washington University School of Medicine
Sebla B. Kutluay: Department of Molecular Microbiology at Washington University School of Medicine
Adrianus C. M. Boon: Department of Medicine at Washington University School of Medicine

DOI: https://doi.org/10.1038/s41467-018-02886-w
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 12

Abstract

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Influenza A virus packaging depends on interactions between nucleoprotein (NP) and viral RNA (vRNA), but the pattern of NP binding is unclear. Using PAR-CLIP, Williams et al. here show that NP binds vRNA non-uniformly and that RNA structures in low-NP binding regions are important for packaging.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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